FastQCFastQC Report
Fri 3 Mar 2023
H7NTWBGXN_n01_DEL2_C24AmpPoolRb1-B_z.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NTWBGXN_n01_DEL2_C24AmpPoolRb1-B_z.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21252646
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG1729230.8136539798385575TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT646270.30408919435255266TruSeq Adapter, Index 6 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGGGGG253990.11950982480016842TruSeq Adapter, Index 6 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)