FastQCFastQC Report
Wed 1 Mar 2023
H7NT7BGXN_n01_7797.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NT7BGXN_n01_7797.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43123538
Sequences flagged as poor quality0
Sequence length151
%GC75

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGGT1924126544.61893873364472TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGGT37218258.630611430815348TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGGG20667224.792561315354042TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGGT19721404.573233300106313TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGTT7635791.770677999564878TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGTGT7083551.642618005971588TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGAT6520051.511946909365368TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGAT6193131.4361368030610104TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT3563640.8263793198044187TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTGT3317150.7692202805808744TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGGG2684090.6224187820581882TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTTCAGAATCGCGGGT2172650.5038199787781792TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGTT1466700.3401158782472811TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGACAGAATCGCGGGT1425030.3304529419640847TruSeq Adapter, Index 15 (97% over 40bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGGT1351110.31331149127884633TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATAGCGGGT1263960.29310211049937507TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGTT1205540.2795549845655057TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCGGAATCGCGGGT1155340.26791401020945915TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTCAGAATCGCGGGT1107150.25673913861149333TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGTAT1099820.255039370841975TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGGA1050250.24354448839517762TruSeq Adapter, Index 15 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGGT1015860.23556972528552736TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACATGTCAGAATCGCGGGT875240.20296108357343037TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTGGGGT844840.1959115692223583TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACATGTCAGAATCGCGGGT823970.19107198486357962TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTCAGAATCGCGGGT818720.18985455228650303TruSeq Adapter, Index 15 (97% over 40bp)
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGGT779300.18071337282205371TruSeq Adapter, Index 15 (97% over 40bp)
GATCTGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGGT765920.17761065893990424TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACATGTCAGAATCGCGGGT745690.17291948540956914TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACATGTCAGAATCGCGGGT700810.16251217606496016TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCATAATCGCGGGT699660.16224550035760052TruSeq Adapter, Index 15 (97% over 39bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACATGTCAGAATCGCGGGT690530.1601283271330845TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGGT677180.1570325700085183TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGAGGGT670590.1555044022593879TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTTAGAATCGCGGGT660610.1531901209033452TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGCT600570.1392673300599779TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACATGTCAGAATCGCGGGT507930.11778486264276368TruSeq Adapter, Index 15 (97% over 40bp)
GATAGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGGT454670.10543429901322104TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCATTCACATGTCAGAATCGCGGGT438820.10175881209004697TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)