FastQCFastQC Report
Wed 1 Mar 2023
H7NT7BGXN_n01_5184.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NT7BGXN_n01_5184.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20796
Sequences flagged as poor quality0
Sequence length151
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTTACATGTCAGAATCGCGGGT10535.063473744950952TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGGT6423.087132140796307TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT4051.9474899019042122TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACATGTCAGAATCGGGGGT1640.7886131948451626TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTTAGAATCGCGGGT1360.6539719176764763TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACATGTCAGAATCTCGGGT1010.4856703212156184TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGGT990.4760530871321408TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACATGTCAGAATCGGGGGG850.4087324485477976TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGGT760.36545489517214846TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCGCGTAT680.32698595883823817TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGTT540.259665320253895TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAAGTCACATGTCAGAATCGGGGT470.2260050009617234Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGACAGAATCGCGGGT470.2260050009617234TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACATGTCAGAATCGCGGTT460.22119638391998464TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTTAGAATCTCGGGT440.21157914983650702TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGTGT440.21157914983650702TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGGGGGG400.19234468166955185TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGAATCGCGGGT390.18753606462781303TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTAACATGTCAGAATCGCGGGT360.17311021350259664TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTGT360.17311021350259664TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCGCGTGT350.16830159646085785TruSeq Adapter, Index 19 (97% over 40bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGGT350.16830159646085785TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGGAT330.15868436237738026TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCGCGGAT330.15868436237738026TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACATGTCAGAATCGCGGAT320.15387574533564147TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACATGTCAGAATCGCGTGT300.14425851125216388TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACATGTCAGAATCTCGGAT290.13944989421042508TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGATAGAATCGCGGGT290.13944989421042508TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATAGCGGGT270.1298326601269475TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACATGTCAGAATCGCGGGG240.1154068090017311TruSeq Adapter, Index 15 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)