FastQCFastQC Report
Fri 6 Aug 2021
H7NLMDRXY_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NLMDRXY_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974592137
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTCCCT21217396121.770538971627264No Hit
CGCATGTG18724195119.21233959226987No Hit
GAAGGAAC18336045518.814070834228367No Hit
TTTCATGA17583316718.041718204422573No Hit
CCTGTGCG415925614.267688956329021No Hit
GGACACGT367489603.770701466268858No Hit
TACTCTTC342953163.518940354430543No Hit
ATGAGAAA293907213.015694451472883No Hit
GGGGGGGG292518003.0014401809194977No Hit
AGGAGAAA24957480.2560812780290264No Hit
TTCATGAA15814400.16226685399576543No Hit
ACGTCCTA14298010.14670762729537598No Hit
GCATGTGA10658180.10936041442739446No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)