FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_nw4-mss.3520000003e133.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_nw4-mss.3520000003e133.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32285802
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG2243900.041.305065225-229
AGGGGGG728500.025.250856225-229
CGGGGGG595100.024.50564225-229
GGGGGGA657350.019.471722225-229
CCGGGGG490450.019.306059225-229
GGGGGGC551500.017.43842225-229
AAGGGGG601700.017.160496225-229
GTATATA405850.016.732891
GTATTAG279450.016.08581
CCCCCCC3643150.015.735772225-229
GTATAGG197350.015.6019021
GAGGGGG745650.015.465705225-229
TGGGGGG573550.015.213151225-229
TATACTG274450.015.20463855
GCGGGGG517650.015.1163435225-229
CCCGGGG474600.015.087459225-229
GTGTATA346300.014.9942821
GGGGGGT375500.014.97135225-229
GTATAAG264150.014.4181821
GTATATG342250.013.5103491
Produced by FastQC (version 0.11.2)