FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_nw3-mss.3520000003e126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_nw3-mss.3520000003e126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41954864
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG3149700.040.665314225-229
AGGGGGG1041250.024.681143225-229
CGGGGGG748750.023.464312225-229
CCGGGGG613900.019.837019225-229
GGGGGGA898250.018.91391225-229
AAGGGGG824600.017.387632225-229
CCCCCCC5112050.017.267973225-229
GGGGGGC746550.016.912102225-229
GTGTATA459800.016.871351
GTATATA490650.016.8164771
GTATAGG231900.016.7027111
ACTGATC466900.016.3591028
CCCGGGG618650.016.250175225-229
GTATTAG337900.016.0037921
TATACTG336400.015.8179165
GAGGGGG1084600.015.146133225-229
CCCCGGG661750.015.059116225-229
GCGGGGG620400.014.675893225-229
GTATAAG321000.014.5734361
TGGGGGG817850.014.49298225-229
Produced by FastQC (version 0.11.2)