FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_53115004-1-mss.3520000003e1e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_53115004-1-mss.3520000003e1e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7239621
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG309600.039.56901225-229
AGGGGGG129300.022.399202225-229
CGGGGGG79250.021.272652225-229
GTATATA114800.018.8815481
GTATTAG55250.017.8683191
GGGGGGA117950.017.151583225-229
GGATCGT19350.016.633998
CCCCCCC541250.016.356422225-229
GTGTATA110750.016.2777231
CCGGGGG67450.015.893736225-229
TATACTG70150.015.7538485
AAGGGGG112350.015.313211225-229
CCCGGGG67950.015.267856225-229
GATCGTT21450.015.00569259
ACTGATC82700.014.7895668
GTATTAT69500.014.5134661
GAGGGGG135000.014.47308225-229
TATACAG80500.014.2614945
GGGGGGC89900.014.136565225-229
GTATAGG37500.014.0214491
Produced by FastQC (version 0.11.2)