FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_52370180-2-rep-mss.3520000003e1d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_52370180-2-rep-mss.3520000003e1d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5593439
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG297900.037.41371225-229
AGGGGGG114300.022.643364225-229
CGGGGGG66350.020.882715225-229
GTGTATA76750.019.153711
GTATTAG41250.018.4690741
GTATATA81100.018.1263541
ACTGATC72300.018.1034938
CCCCCCC432600.017.82738225-229
TATACTG49800.017.4511365
CCGGGGG54750.017.189783225-229
GTATAGG33800.017.1430381
GTCTTAG37150.016.1748391
TGCACTG103450.015.971965
GTCTAAG34800.015.7253981
GGGGGGC74400.015.460793225-229
CTGATCA77300.015.4060779
GTATTAT56150.015.288011
CCCCCCG80150.015.112699225-229
GTCTATA38400.015.0894441
GTGTTAG51400.015.030731
Produced by FastQC (version 0.11.2)