FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_52370180-2-mss.3520000003e183.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_52370180-2-mss.3520000003e183.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5338667
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG356500.041.453773225-229
AGGGGGG130100.025.123161225-229
CGGGGGG68000.023.6477225-229
CCGGGGG58250.020.854456225-229
CCCCCCC526350.019.29352225-229
TATACTG50050.018.8640565
GTCCTAG32700.017.719661
CCCGGGG58800.017.686813225-229
GGGGGGA115050.017.5471225-229
AAGGGGG112950.017.099604225-229
ACTGATC71350.016.990758
GTGTATA74800.016.9273821
GTATAGA48300.016.884021
GTATTAG44250.016.2469161
GTATATA87300.016.2244151
CCCCCCG85200.016.104233225-229
GAGGGGG132100.015.480786225-229
CTATACT44400.014.9819824
CCCCGGG60250.014.795278225-229
CTGATCA75550.014.7681779
Produced by FastQC (version 0.11.2)