FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_52260024-1-mss.3520000003e1c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_52260024-1-mss.3520000003e1c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5366567
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG301950.039.40424225-229
AGGGGGG118900.022.318956225-229
CGGGGGG67700.022.30692225-229
GTATAGG31250.021.6340871
GTATTAT53850.019.5293771
ACTGATC75900.019.2274138
GTATATA77550.018.819371
GTGTATA69400.017.6276071
CCGGGGG55650.017.254787225-229
GGGGGCG45600.017.037558225-229
CCCCCCC496650.016.767954225-229
CTGATCA81050.016.41719
TGCACTG108050.016.3865285
GGGGGGC78500.015.79068225-229
GCACTGA92750.015.7349326
GCGGGGG51150.015.700892225-229
GGGGGGA108850.015.477863225-229
TATACTG49850.014.8529165
GTATAGA48500.014.8245351
GTATAAG42150.014.7665291
Produced by FastQC (version 0.11.2)