FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_52150223-1-mss.3520000003e1b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_52150223-1-mss.3520000003e1b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6305116
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG204750.034.820374225-229
GTCCTAG33300.022.2360551
CGGGGGG57550.020.345762225-229
AGGGGGG106700.020.240463225-229
GTCCTAA43950.017.824481
GTCCTGG58600.017.5803091
GTCCTAC30050.015.7130711
GGGGGGA100300.015.392337225-229
TCCTAGG52300.015.3882192
TGCACTG96450.015.0194275
GTGTATA93950.014.7348531
AAGGGGG94850.014.630803225-229
GCGGGGG43700.014.488618225-229
GTCTAAG43250.014.14300251
GTCCTAT33700.014.011211
TATACTG69900.013.9696685
GTATATA118900.013.8992621
CTCCTAT70350.013.7287591
TGCTATG63650.013.6557452
CTCCTAA70200.013.60522651
Produced by FastQC (version 0.11.2)