FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_50331580-1-mss.3520000003e1ad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_50331580-1-mss.3520000003e1ad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4070112
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG181700.037.61983225-229
AGGGGGG84150.020.908678225-229
CGGGGGG44900.017.437908225-229
ACTGATC50900.017.0766548
GCGGGGG37700.017.03461225-229
GTCCTAG23950.016.132311
GGGGGGA79450.016.055536225-229
GTATTAT49750.015.9638731
TATACTG39900.015.5991655
TGCACTG72000.015.2024615
CCCCCCC280800.014.912847225-229
AAGGGGG72700.014.88406225-229
GAGGGGG88950.014.736388225-229
GTCTCGC15450.014.5878221
GTGTATA54850.014.2838691
GTATAAG38100.014.0846031
GTATATA71700.013.7710891
GTGTAAG42150.013.7497811
GTATAGT32650.013.4772191
GTATTAA50200.013.4690331
Produced by FastQC (version 0.11.2)