FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_50220371-2-mss.3520000003e190.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_50220371-2-mss.3520000003e190.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7716688
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG369600.038.491947225-229
CGGGGGG86200.023.03378225-229
AGGGGGG161800.022.355278225-229
CCGGGGG71650.020.120783225-229
GTATTAG59100.017.2511441
GTATATA112700.015.9980531
GTATAGG41350.015.5724711
GGGGGGA147150.015.018422225-229
CCCGGGG77950.014.729232225-229
GTGTATA92500.014.6187611
CCCCCCC565700.014.588691225-229
GTCTTAG51200.014.4630841
GGGGGCG60400.014.435407225-229
AAGGGGG141250.014.3623225-229
TATACTG73450.014.1708865
GTCTAAG52750.014.0381031
CCCCCCG106150.013.662616225-229
GAGGGGG173500.013.633133225-229
GTATAAG66700.013.3546971
GCGGGGG65300.013.352199225-229
Produced by FastQC (version 0.11.2)