FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_44065-mss.3520000003e176.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_44065-mss.3520000003e176.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5622068
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG366950.044.039364225-229
AGGGGGG130050.028.12841225-229
CGGGGGG69900.022.981703225-229
GGGGGGA111050.019.811779225-229
AAGGGGG110250.019.005276225-229
CCGGGGG52100.018.097874225-229
CCCCCGG64200.017.81467225-229
CCCGGGG56850.017.200026225-229
GTGCTAG42750.017.0660271
CCCCCCC491850.016.774155225-229
GAGGGGG138400.016.716728225-229
GGGGGGC80050.016.686745225-229
GTATATA96300.016.600441
GTCCTAG34050.016.0698811
GTCCTAT34150.016.0228251
TGGGGGG110450.015.334779225-229
GCGGGGG54350.015.260388225-229
GGGGGCG47450.015.087566225-229
CCCCCCG79800.014.988536225-229
GTGTATA85900.014.9881381
Produced by FastQC (version 0.11.2)