FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_40255-mss.3520000003e140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_40255-mss.3520000003e140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4332381
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG271950.037.43651225-229
AGGGGGG108150.022.57645225-229
CGGGGGG55000.020.8345225-229
GTGTATA63150.019.7103481
CCGGGGG43150.018.793577225-229
GTATATA70250.017.8710161
GTATTAG38300.017.6502741
AAGGGGG89050.017.619293225-229
GGGGGGA96800.017.210316225-229
TATACTG41000.015.9615645
ACTGATC54100.015.8634328
TCTGTCG21050.015.7984318
CTGATCA59500.015.6859819
CCCCCCC407950.015.535469225-229
GATCGTT16600.015.5100299
CCCGGGG42350.015.193991225-229
GTATTAT47450.015.1512421
CTGTCGC19850.015.1323699
GTACGGA16400.015.0485171
TCTATAC41200.014.8430163
Produced by FastQC (version 0.11.2)