FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_35008-2-mss.3520000003e169.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_35008-2-mss.3520000003e169.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5165618
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG319000.041.38842225-229
AGGGGGG122800.024.438585225-229
CGGGGGG63200.023.67333225-229
CCGGGGG50000.021.348637225-229
GTATATA84750.018.9903851
AAGGGGG101250.018.319818225-229
CCCCCCC451000.017.925686225-229
GGGGGGA101900.017.687782225-229
ACTGATC57300.017.0379968
GTGTATA75650.017.0198021
CCCGGGG50500.016.805857225-229
TATACAG53750.016.7670965
GAGGGGG120200.016.74186225-229
GGGGGGC70100.015.726528225-229
TATACTG50950.015.5827665
CCCCCCG74850.015.196092225-229
GTATAGG28650.014.9801991
GTACTAG25150.014.9318041
CTGATCA62750.014.874479
CCCCCGG57750.014.847538225-229
Produced by FastQC (version 0.11.2)