FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_35008-1-mss.3520000003e15d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_35008-1-mss.3520000003e15d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5027894
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG234750.040.366245225-229
CGGGGGG51100.024.404505225-229
AGGGGGG104700.023.066864225-229
GTATTAG43900.017.5976121
GGGGGGA94900.017.212711225-229
GTATATA84200.016.0562551
GCGGGGG40600.015.790627225-229
CCGGGGG38050.015.694838225-229
AAGGGGG90500.015.623554225-229
GGGGGGC60200.015.463646225-229
TGGGGGG78600.015.307369225-229
GAGGGGG108550.014.401003225-229
CCCCCCC365550.014.222549225-229
GTGTATA69800.013.9885191
TATACTG53400.013.8633355
GTATATG61700.013.7381321
GTATAGG30800.013.5862521
CCGCGGG11709.094947E-1213.511053225-229
GTCTAAG33000.013.3307831
GGGGGCG34600.013.198658225-229
Produced by FastQC (version 0.11.2)