FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_34310-1-mss.3520000003e119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_34310-1-mss.3520000003e119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8667500
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG512500.041.669914225-229
AGGGGGG195400.026.440403225-229
GGGGGGA158650.020.495905225-229
CCGGGGG79950.019.850214225-229
AAGGGGG154350.019.166964225-229
CGGGGGG107400.019.113424225-229
GTATATA138650.018.649691
ACTGATC94200.018.4480678
TATACTG76100.018.044185
CCCCCCC637900.017.929028225-229
GGATCGT28150.017.5293648
GTGTATA135400.017.4332561
GTACGGA35100.017.4237731
CCCGGGG83000.016.834599225-229
GATCGTT31600.016.2947779
GTATAGG49500.016.256631
CTGATCA100650.015.8805949
GAGGGGG205550.015.735467225-229
CCCCCCG109550.015.352816225-229
AGGATCG33300.015.1420777
Produced by FastQC (version 0.11.2)