FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_34302-1-mss.3520000003e10c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_34302-1-mss.3520000003e10c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5431563
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG338050.038.88252225-229
AGGGGGG126400.025.255505225-229
GGGGGGA108350.021.976362225-229
CGGGGGG65450.020.522635225-229
GTATTAG45050.018.8148271
CCCCCCC482500.018.583088225-229
AAGGGGG102550.018.541416225-229
TATACTG50950.017.4763135
CCCGGGG47850.017.134363225-229
CCGGGGG47800.016.969814225-229
GTGTATA78000.016.7815911
GTATTAT53800.016.7519321
GTATATA85200.016.7486551
GCGGGGG46400.015.978022225-229
GGATCGT18300.015.8270738
ACTGATC61800.015.7957728
GAGGGGG130850.015.597829225-229
TCTATAC43800.015.4308663
CTATACT42800.015.2898064
GTATAAG43250.014.8844591
Produced by FastQC (version 0.11.2)