FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_34298-1-mss.3520000003e0fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_34298-1-mss.3520000003e0fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5474425
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG229700.038.149586225-229
AGGGGGG108150.021.768173225-229
TATACTG62200.020.8719795
CTATACT53750.019.761754
CGGGGGG51350.019.527298225-229
GTCCTAG31050.018.3166641
GTGCTAG34950.017.8079091
GTATTAG44800.017.7249811
GTATATA106050.017.7075921
GGGGGGA97950.017.26959225-229
GGATCGT16800.016.60348
ACTGATC64300.016.1842928
AAGGGGG92850.015.588738225-229
GTCCTAA35900.015.2443131
GTATAGG34550.015.2187911
GCGGGGG40550.015.05192225-229
GTCTAAG39000.014.8580311
CTGATCA67300.014.5065199
GTATTAT62150.014.5034151
GATCGTT18250.014.1086449
Produced by FastQC (version 0.11.2)