FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_34274-2-mss.3520000003e0e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_34274-2-mss.3520000003e0e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6380073
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG383750.038.480564225-229
AGGGGGG152100.022.642176225-229
CGGGGGG85550.020.534472225-229
GTATATA108050.018.6673071
TATACTG63850.017.809125
CCGGGGG65500.016.994987225-229
AAGGGGG121500.016.677534225-229
GGGGGGA135850.016.452263225-229
ACTGATC76150.016.2000088
GTATAGG39250.016.1272721
CCCCCCC530000.015.703203225-229
GTATTAG53400.015.4702761
GTATTAT68250.015.090971
GTGCTAG44600.014.9143881
TCTATAC56800.014.5429833
CCCCCCG92150.014.533745225-229
GCGGGGG64950.014.460949225-229
GTCCTAG40850.014.4450551
GAGGGGG168450.014.30082225-229
GTGTATA96300.014.1490091
Produced by FastQC (version 0.11.2)