FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_34271-1-mss.3520000003e0d4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_34271-1-mss.3520000003e0d4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4420864
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG256600.040.18424225-229
AGGGGGG102750.022.262623225-229
CGGGGGG54550.019.837873225-229
GGGGGGA89850.018.212923225-229
ACTGATC57350.017.5853548
TATACTG44900.017.2047275
CCCCCCC390000.016.896704225-229
AAGGGGG85350.016.286898225-229
CCGGGGG42750.016.258324225-229
GTATAAG35400.016.0650961
GTATATA70200.016.0495511
GTATTAG37850.015.87571
CTGATCA62950.015.16896259
GGATCGT12801.8189894E-1215.0875678
GAGGGGG111300.015.019076225-229
GTCCTAG29100.014.7495151
GTGTATA59750.014.7260641
GTCGTAG5851.1772104E-414.6738761
GTATTAT51050.014.5032091
GTATTAA51000.014.3070291
Produced by FastQC (version 0.11.2)