FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_34211-2-mss.3520000003e225.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_34211-2-mss.3520000003e225.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6263952
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG281950.040.951073225-229
AGGGGGG109000.024.164585225-229
CGGGGGG66200.020.741737225-229
CCCCCCC417050.019.361076225-229
AAGGGGG88300.019.071283225-229
CCGGGGG47550.018.343275225-229
GTCCTAA39700.017.2988411
TGCTAGA70650.016.7056352
GTGCTAA41400.016.5885011
GTCCTAT36550.016.1474081
GTGCTAG39450.016.048431
GGGGGGA101950.016.009605225-229
GTATATA128750.015.33550551
GTCCTAG34350.015.3072391
TCCTAGA66400.015.0278652
TCCTATA69950.014.8787512
GCGGGGG46050.014.8150625225-229
GAGGGGG111250.014.748898225-229
TATACTG66550.014.6709575
GCTAGAC53750.014.5720243
Produced by FastQC (version 0.11.2)