FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_34211-1-mss.3520000003e218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_34211-1-mss.3520000003e218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6793022
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG392900.038.173763225-229
AGGGGGG133950.022.613178225-229
CGGGGGG85100.021.599678225-229
GCTAGAC57400.019.809793
CCGGGGG64250.019.207026225-229
GTCCTAG43500.018.4966491
ATGCTAG57650.018.2367671
GGGGGGA116800.017.880093225-229
AAGGGGG107300.017.854614225-229
CCCCCCC578100.017.764017225-229
CCCCCGG71250.017.683376225-229
TGCTAGA77450.017.451592
GTCCTAT38450.017.2988191
CCCCCCG90950.016.699642225-229
CCCGGGG66450.016.623108225-229
ACTGATC81100.016.4006548
CTATACT60800.016.0551414
TATACTG68350.015.8512085
CCCCGGG68100.015.713458225-229
GTATTAG56700.015.704211
Produced by FastQC (version 0.11.2)