FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n02_34139-1-mss.3520000003e20b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n02_34139-1-mss.3520000003e20b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5426245
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGG293000.037.62795225-229
AGGGGGG107700.024.66202225-229
CGGGGGG66000.024.410252225-229
GTATTAG43400.018.5423261
CCCCCCC479300.018.078407225-229
CCGGGGG51850.017.9713225-229
GGGGGGA97900.017.345882225-229
GTACTAG24300.017.220711
AAGGGGG95750.016.371113225-229
TATACTG49550.016.2388135
GTATATA87900.016.235121
ACTGATC72700.016.2322588
GTATAAG45400.015.8347661
ACGGGGG36600.015.465955225-229
GTATAGA53350.015.2852271
GTATAGG29950.015.0468361
GGGCGGG49600.014.92388225-229
TCCGTAC17450.014.7555873
GCACTGA88800.014.4978956
GCGGGGG47350.014.345613225-229
Produced by FastQC (version 0.11.2)