FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_nw4-mss.3510000003e136.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_nw4-mss.3510000003e136.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32285802
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA455600.016.3513471
GTATTAG310550.015.9003671
GTGTATA396950.015.8657641
TATACTG315150.014.4591775
GTATAGG207350.014.0639271
GTATAGA280650.013.9270551
GTATAAG294200.013.8686841
GTATTAT402350.013.8561011
TATACAG380550.013.4871525
TACACCG199250.013.09481055
GTATAAT325850.012.9728391
GTCTAAG239350.012.9515211
GTGTAGG288150.012.9267271
TATATAC483200.012.8785883
TACACTG401050.012.2479615
GTATTAA379800.012.0011171
TAGACAG791250.011.8276265
TCTATAC291700.011.8005253
GTATAGC199100.011.6311911
GTATAAA484900.011.4972211
Produced by FastQC (version 0.11.2)