FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_nw3-mss.3510000003e129.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_nw3-mss.3510000003e129.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41954864
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG255400.016.5992621
GTATAGA344300.016.5125661
TATACTG376800.016.0593515
ACTGATC586600.015.9328868
GTGTATA495350.015.88021851
GTATATA545300.015.571531
GTATTAG377100.015.23877051
GTGTAGG361900.013.6442621
TATACAG461950.013.2582745
TACACTG540950.013.1562155
TATATAC592200.013.1351143
GTATAAG338700.013.0956481
GTGTAAG418450.012.9716051
GTCTAAG318000.012.792181
CTGATCA677850.012.6856999
GTGTAGA441800.012.6465691
GTATTAA416550.012.589551
GTCTTAG352550.012.3378991
GTATTAT429050.012.2513241
GCACTGA779750.012.2375376
Produced by FastQC (version 0.11.2)