FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_53115004-1-mss.3510000003e1e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_53115004-1-mss.3510000003e1e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7239621
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA121750.018.7181051
TATACTG70900.018.1409055
GTGTATA113150.016.5674171
GTATTAA68900.016.1822931
GGATCGT22800.015.5804188
GTATTAT70400.015.3153861
GTATTAG61050.015.2526681
GTATAAG63800.014.9793121
GTATAGG45050.014.9584241
TAATACT80650.014.8846174
GTCCTAG49800.014.7618181
ATATACT95200.014.6684364
TAGTACT48650.014.6036394
GTATAGA63900.014.5723851
GTGTAAA119750.014.5288331
GATCGTT25400.014.4676329
GTATAAT69450.014.2899491
GTCTTAA66750.013.9501941
TATATGT137500.013.8084514
GTGTAAG74700.013.6136761
Produced by FastQC (version 0.11.2)