FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_52370180-2-rep-mss.3510000003e1d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_52370180-2-rep-mss.3510000003e1d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5593439
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC97300.019.0099288
CCGTACC20150.018.8454674
TCCGTAC22100.017.7373833
TATACTG55650.016.72885
GTACGGA23000.016.5154341
ACGGACC32300.016.307455245
CTGATCA102350.016.2764479
CGTACCA23550.016.1245335
GTATAAG44450.015.9886711
GGATCGT15450.015.8568848
TATAATA80950.015.7379562
GTGTATA86150.015.5034131
GTATAGA47050.015.3655651
GTATATA89650.014.89814951
GTGTAGA56800.014.8852761
TACACTG80850.014.8478095
TATACAG70000.014.6993315
GTATTAG46900.014.3696421
GATCGTT16400.014.19117459
GTCCTAG40650.014.1675071
Produced by FastQC (version 0.11.2)