FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_52370180-2-mss.3510000003e186.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_52370180-2-mss.3510000003e186.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5338667
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG58050.017.3029525
GTATTAG50350.016.0622961
CGACGAA5504.5068818E-415.590213245
TCTATAC50450.015.29735853
GTGTATA86900.015.2288521
GTATAAG52100.014.5820021
GTATAGG34300.014.2898951
ACTGATC86250.014.0599798
GTGCTAG48850.013.7962571
GTATATA99350.013.567131
GTGTACG10051.4881753E-613.4118841
GTCCTAG42350.013.3096791
GTATTAT63600.013.2939841
GTACTAG30100.013.0270671
CTATACT52600.012.8081234
GTATAGA46150.012.7447911
GTGTAAG63850.012.6661961
GTGTTAG64050.012.6266451
CTTACAC66250.012.5736133
GTCTTAG45000.012.52588751
Produced by FastQC (version 0.11.2)