FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_52260024-1-mss.3510000003e1c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_52260024-1-mss.3510000003e1c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5366567
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA79350.019.3018151
GTATTAG48500.018.4423481
GTATAGG32950.017.1055681
GTATATA89600.017.093741
TATACTG52650.016.9839295
CTATACT47750.016.6746864
GTATAGA42800.016.604251
GTGTAGG46050.016.2306251
ACTGATC99300.016.0364348
TACACTG80300.015.712145
GTGTAGA57750.015.7005471
GTATAAG46600.014.9873211
TATACAG64050.014.9172635
TCTATAC49700.014.7883823
ACTCGCG10851.5057594E-814.67666158
GATCGTT18450.014.6061559
GGATCGT17650.014.5743188
CGCGACC7602.1729657E-514.506101245
GTATAGC27900.014.4925511
GTATTAT56250.014.376611
Produced by FastQC (version 0.11.2)