FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_52150223-1-mss.3510000003e1bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_52150223-1-mss.3510000003e1bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6305116
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG44550.019.2514611
GTGCTAG52700.017.9016651
GTATTAG59450.017.1056521
GCTAGAC70750.016.448953
GTATATA121500.016.4370821
ATGCTAG62400.016.100621
TACACTG92900.015.1633645
TATACTG71500.014.9048195
GTGTATA108850.014.5202681
CTCCTAT87400.014.2988831
ACTGATC94450.014.2660658
GTATAGA56900.014.2116671
GTATAGG35100.013.9625981
TCTATAC60550.013.9596963
TGCTAGA93350.013.9101732
CTATACT63800.013.8239524
CCTATAC45500.013.7309263
GTGCTAT60000.013.68161
GTCCTAA51900.013.4561511
TATACAG81250.013.4177635
Produced by FastQC (version 0.11.2)