FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_50331580-1-mss.3510000003e1a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_50331580-1-mss.3510000003e1a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4070112
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT14200.021.565838
GATCGTT14950.020.4839329
GTATATA74100.017.0310041
GTACGGA11709.094947E-1216.755421
CGACGAA4450.001976566416.516035245
AGGATCG19950.015.964127
TATACAG50250.015.8449855
GTATAGG22550.015.2136261
GTGTAGG33950.014.7967161
GTATAGC21800.014.6129571
TATACTG40300.014.5898465
GTATTAG39600.014.5419931
TACACAG95800.014.4486565
TCCGGTA5958.182955E-414.4114032
GTGTATA64900.013.9703821
TACACTG60150.013.6443785
TATATAC74950.013.56537153
GTATTAA45550.013.4493991
CCTATAC31000.013.0399963
GTATAAA69950.012.9617981
Produced by FastQC (version 0.11.2)