FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_50220371-2-mss.3510000003e193.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_50220371-2-mss.3510000003e193.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7716688
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG45900.020.2879261
GTATATA118100.017.11871
ACTGATC101750.015.7705558
GTGTATA100850.015.4299521
GTCGTAG7301.4815925E-515.1062411
TATACTG73850.015.09388355
CTAGGAC72700.014.8275573
GTGTAAG78900.014.7531011
TACACTG108000.014.2908115
GTCTATA58600.014.2183111
GTATAGA57750.014.2154151
GTATTAG70200.014.1378921
TATATAC127550.013.9254383
GTATAAT75800.013.739991
GTACCGT9057.4755662E-613.5351016
GTATTAT75950.013.3901991
TATATGT136700.013.1722174
TATAGTG59800.013.1095845
GTATAAG61200.013.0136361
TAGACAG245900.012.9516435
Produced by FastQC (version 0.11.2)