FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_44065-mss.3510000003e179.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_44065-mss.3510000003e179.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5622068
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC24750.023.261429245
GTATAGG32850.017.157731
ACTGATC87450.015.9681628
GCTAGAC58350.015.9547633
GGATCGT19300.015.2322428
GTCCTAG46800.015.1851641
GTGTATA102700.015.1520231
CTATACT58400.015.1019624
CCTATAC40650.015.0670093
TACACTG90100.014.8187515
CTACACT72800.014.80693154
TATACTG60850.014.4937855
CTGATCG10251.1943848E-714.3404719
CGCTAGG6109.881136E-414.0567792
GTATATA113350.013.8364861
ATGCTAG63700.013.657031
CCTACAC56900.013.5626933
GTCCTAC32850.013.4277891
CCGCTAG7302.5008272E-413.4277891
GATCGTT19950.012.8938459
Produced by FastQC (version 0.11.2)