FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_40255-mss.3510000003e143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_40255-mss.3510000003e143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4332381
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG41050.019.1026131
TATACTG46850.017.5176535
GGATCGT19050.016.0751768
TACACTG61300.015.786195
ACTGATC73900.015.58095558
TATACAG57550.015.5377535
GATCGTT19950.015.3499799
GTATATA80700.015.182821
GTATAGA39450.014.5974451
GACGACC6751.2893147E-414.517838245
TATATAC80700.014.2683923
GTGTAGA46250.014.0407441
AGGATCG23250.013.6981247
TCCGTAC13551.3897079E-913.5604063
GTGTAAG50550.013.3311461
GTATAAT47900.013.3012911
GTGTAGG35250.013.2084081
ATATACT63600.013.0967184
TAGTACT30950.013.0606264
GTATTAT51600.013.0598731
Produced by FastQC (version 0.11.2)