FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_35008-2-mss.3510000003e16c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_35008-2-mss.3510000003e16c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5165618
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA86400.017.8692321
GTATAGG30150.017.0691171
GTATATA99100.016.8156761
ACTGATC78900.015.5250628
CCGTACC17600.015.3115934
TCCGTAC19500.015.0761753
TATACAG65100.015.0528725
TATACTG52500.014.4658095
GTATTAA52400.014.2642071
GTGTAGG40450.014.2373271
GTATTAG47500.014.18790151
GTATAAT53700.014.1470711
CGTACCA19100.014.1091115
TCTATAC48850.014.0422923
GTATAGC25000.013.7235711
TATATGT112050.013.6649654
GTGTAGA54250.013.5519131
CCGTAGA8254.7062702E-513.3629732
GTCTATA40450.013.3285611
GACCGTG12156.652772E-813.1062197
Produced by FastQC (version 0.11.2)