FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_35008-1-mss.3510000003e150.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_35008-1-mss.3510000003e150.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5027894
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA47300.018.1323031
GTATTAG46100.017.2754151
TATACTG54650.016.8106125
GTATATA90100.016.318211
TGTACGG6055.066384E-516.1974645
GTGTATA79800.016.1213871
GTATTAT60400.014.808191
GTGTAGA53800.013.8919621
GCGTAGT7101.97703E-413.8053591
GTATAGG28000.013.5649991
TCTATAC48950.013.5131863
GTATTAC35150.012.8971111
GATCGCG9651.448158E-512.6936088
GTCTTAA53300.012.6430411
GTATAGC27750.012.3626371
GTGCTAG44600.012.3621421
GACTGCG10903.7134942E-612.36171157
GTATTAA55950.012.2632061
GTATAAG45100.012.2250881
CTATACT49100.012.2243844
Produced by FastQC (version 0.11.2)