FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_34310-1-mss.3510000003e11c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_34310-1-mss.3510000003e11c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8667500
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG49050.020.2336461
GGATCGT29750.019.3519088
GTGTATA137300.019.1865161
GTATATA155250.019.0990561
GATCGTT32900.018.2434969
TATACTG87100.017.5794285
GTATAAG70300.017.4290141
ACTGATC117050.016.5347848
AGGATCG37350.016.3980527
GTCTATA64400.016.3621641
GTATTAT77500.016.2840981
GTACGGA36600.015.7342091
GTATAGA76250.015.5869111
GTATTAA78850.015.5391211
GTATTAG69550.015.32675651
GTATAAT82050.014.7837561
TCCGTAC36300.014.5108313
GTGTAGG59350.014.2448051
GTTGTAG67600.014.1376121
TTAGACA133050.013.810184
Produced by FastQC (version 0.11.2)