FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_34302-1-mss.3510000003e10f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_34302-1-mss.3510000003e10f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5431563
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT22650.024.3361668
GATCGTT24700.022.316369
AGGATCG27600.019.5277167
GTGTATA84950.018.4616451
TATACTG55250.016.4061725
GTATTAT56250.016.3366321
GTACCGT6908.709563E-615.9772226
GTATTAG47350.015.7846031
GTATAAG47650.015.4280891
GTATATA93900.015.2666621
GTGTAAA99550.015.2617461
GTATAGG30150.014.6298191
CTATACT49150.013.9563664
GTATAGA50400.013.8569651
GTCCTAA40450.013.6306891
GTATTAC31500.013.6138591
GTATTAA51400.013.5873741
GTATAAT51500.013.560991
GTCTAGG53500.013.5120741
TATACTA54550.013.4730015
Produced by FastQC (version 0.11.2)