FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_34298-1-mss.3510000003e0f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_34298-1-mss.3510000003e0f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5474425
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA2700.00216099522.683931245
TATACTG62000.020.3498675
CTATACT52900.018.0617124
ACTGATC89000.016.6537258
GTATATA113850.016.251851
GTGCTAG44500.015.6954471
GTGTATA100500.015.6064021
GCTAGAC57950.015.4312583
CCTATAC39300.015.273393
TATACAG73350.015.1969815
GTCCTAA41600.015.0222761
GTATTAG53950.014.9903351
CTGATCA96450.014.9860749
GTATTAT65300.014.6366031
GTATTAC39400.014.6170761
TCTATAC53900.014.5453143
GTGTAAG62000.014.4274651
GTATAAG50150.014.1715041
TACACTG80600.014.1339635
GTATTAA60050.014.079751
Produced by FastQC (version 0.11.2)