FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_34274-2-mss.3510000003e0e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_34274-2-mss.3510000003e0e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6380073
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG71400.018.0141745
GTATATA116800.017.9391771
CTATACT61400.016.3597094
GTATTAG63000.016.1430931
GTCGTAC4850.00350253515.1585871
ACTGATC102800.015.0141498
GTATTAT67100.014.97409251
GTGTATA102550.014.935631
TATACAG83700.014.3424685
GCTATAC33750.014.1555813
GTGCTAG56750.014.0344921
GTGTAGA66400.014.0247371
GTCCTAT42000.014.0036481
CCTATAC47250.013.7407663
TACACTG98400.013.6937015
GTATAAT65050.013.5623331
GTATAGA57750.013.3671191
CTTACAC80000.013.3218983
GTGTAAG69950.013.3129741
TTATACT75900.013.0729414
Produced by FastQC (version 0.11.2)