FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_34271-1-mss.3510000003e0d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_34271-1-mss.3510000003e0d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4420864
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG46650.017.5929325
GTATAGG27200.017.1166291
GTGTATA69150.016.8319681
CTGCGCG5504.5071798E-415.5899789
GTGTAGG37600.015.31487751
GTCGTAA5655.5205193E-415.1793471
GTATATA81250.015.0792641
ACTGATC69400.015.0028768
TCTATAC40800.014.7114653
GTCCTAT28500.014.6163031
CTATACT44150.014.4273874
CGGTACT5958.184594E-414.4110684
GGATCGT13651.0186341E-1014.3582788
GTATAGC21600.014.1804431
GTATTAT52600.013.5097021
TGCACTG108650.013.3035445
CCTACAC48850.013.2901943
GTATAAG39150.013.1438021
TATACAG54750.012.97654155
TAATAGT46900.012.7978894
Produced by FastQC (version 0.11.2)