FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_34211-2-mss.3510000003e228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_34211-2-mss.3510000003e228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6263952
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT20500.023.303428
GTCCTAC31550.021.3608171
GATCGTT23550.021.325639
AGGATCG25700.020.01827
GTCCTAG42350.019.6747881
GTCCTAA46550.018.6893061
TATACTG78100.017.2524415
CCTATAC47000.016.4199713
ATGCTAG66500.016.3992081
CTCGCGT12851.8189894E-1216.205219
CCCTATA40950.016.1536082
GTGCTAC35850.015.7225581
GCTAGAC66500.015.6576643
TGCTAGA95200.015.3122762
ACTCGCG13655.456968E-1215.2554568
CCTAGAC65950.014.8595253
GTGCTAG45750.014.462961
TCTATAC66000.014.1058543
ACTGATC90050.014.0107838
TTAGACA113800.013.99319654
Produced by FastQC (version 0.11.2)