FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_34211-1-mss.3510000003e21b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_34211-1-mss.3510000003e21b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6793022
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC33000.021.900526245
CTCGCGT10800.019.2812549
GTCCTAA53250.018.4080751
GTCCTAG47250.017.8930871
ACTCGCG11056.366463E-1116.6280868
CCTACAC72950.016.6243843
GTCCTAC34150.016.1458071
TCCTACG12951.8189894E-1216.0810662
TGCTAGA98350.016.0675982
TATACTG75850.015.9879555
CCCTACA82500.015.7393792
GCTAGAC70250.015.6939353
GTGCTAG54750.015.6657761
ATGCTAG69450.015.1727471
CTACACT80700.014.7236224
GTGTATA120500.014.642441
CTATACT66150.014.6289694
ACTGATC98600.014.5352418
GTCCTAT41950.014.3120581
CCGGACC14652.0008883E-1114.2143755245
Produced by FastQC (version 0.11.2)