FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l02n01_34139-1-mss.3510000003e20e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l02n01_34139-1-mss.3510000003e20e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5426245
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG32400.017.3953821
GTGTATA91400.017.1587221
CGCGAAA5052.3520441E-416.9793245
ACTGATC91300.016.6364258
GTCGTAG5202.9350325E-416.4936161
GTATAGA50900.016.3686281
GGATCGT21550.015.9154848
GATCGTT22600.015.7180499
TACGCTA6509.351245E-515.0759875
GTATTAT58150.014.9599381
TATAGTG45050.014.9546765
GTATATA105000.014.819571
GTATTAG51300.014.807971
GTATTAA56900.014.6425871
TATACTG56250.014.5902045
GTCCTAA42300.014.4827421
TACACTG82700.014.3673075
CCGTACC16350.014.2348524
CTGATCA102800.014.1795679
AGGATCG27150.013.9862397
Produced by FastQC (version 0.11.2)