FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_nw2-mss.3520000003c7fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_nw2-mss.3520000003c7fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46442919
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG1492600.0163.31691245
GGGGGGG3259300.074.8079235-239
GGCGGGG721800.063.46776245
CGAGGGG369550.061.98218245
GTAGGGG401150.046.886684245
GGAGGCG553200.045.44504245
CCACCCC1821950.044.461872245
CGGGGGG787350.041.782993235-239
AGGGGGG1036600.041.66872235-239
GGAGGGA1115150.040.74661245
GAAGGGG1080250.040.425323245
GGACCCC489050.036.36512245
GGAGGGC678300.036.102634245
TTAGGGG331750.035.364258245
CCGGGGG630750.035.117977235-239
GGAGGAA1211200.033.59468245
GCAGGGG912200.033.48025245
AGAGGGG1111400.031.584637245
GGTGGGG1040400.031.413164245
GGAGGGT542650.030.542538245
Produced by FastQC (version 0.11.2)