FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_nw1-mss.3520000003c7b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_nw1-mss.3520000003c7b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43169337
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG1155350.0133.47319245
GGGGGGG1993150.071.7591235-239
GGCGGGG462650.059.32216245
GTAGGGG310200.050.600246245
CGAGGGG264050.042.410362245
GGAGGCG392200.040.25494245
AGGGGGG778250.040.006496235-239
GAAGGGG883950.038.00596245
CGGGGGG456150.036.972095235-239
CCACCCC1603450.036.866234245
GGAGGGA953050.036.502438245
AGAGGGG798400.033.915623245
GGAGGAG975600.031.895267245
TTAGGGG277800.031.7203245
CCGGGGG360350.029.982037235-239
GGAGGAA1041700.028.81399245
GGTGGGG711900.028.366257245
GGAGGGC485750.027.967089245
GCAGGGG710150.027.919136245
GGGGGGA674800.026.97489235-239
Produced by FastQC (version 0.11.2)