FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_50330665-2-mss.3520000003c882.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_50330665-2-mss.3520000003c882.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6354890
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG181900.093.27791245
GGGGGGG287250.063.06015235-239
GGCGGGG65200.061.231514245
CGAGGGG34900.041.59723245
GAAGGGG137200.039.679577245
CGCGGGG21050.038.79362245
GTAGGGG47950.037.845238245
CGGGGGG67850.036.133747235-239
CGAGCCG10100.002125570635.934242245
AGAGGGG125700.035.369644245
AGGGGGG125400.035.22382235-239
ATAGGGG46600.031.153292245
GGCGGCG21903.7709287E-629.001722245
GGACGGG45400.027.979637245
CGAGGCC29804.8790753E-827.402876245
GGCGGCC26655.6277895E-727.237211245
GGAGGGC83850.027.052464245
TTAGGGG47150.026.941156245
CGCGGGC13551.1037264E-726.784935245
CCAGGGG102550.026.543333245
Produced by FastQC (version 0.11.2)