FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_50330665-1-mss.3520000003c875.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_50330665-1-mss.3520000003c875.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6597542
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG195300.0101.72505245
GGGGGGG314250.064.11758235-239
GGCGGGG72950.053.472343245
CGAGGGG40600.046.922302245
GTAGGGG53400.042.470245245
CGGGGGG74650.039.645058235-239
GGAGGCG62000.038.04238245
AGGGGGG133350.036.938503235-239
TTAGGGG48650.033.56415245
GAAGGGG147050.030.228535245
AGAGGGG127850.029.801258245
GGAGGGA176800.029.246819245
CCGGGGG58300.029.206316235-239
GTAGGCG16106.1031757E-428.172812245
GGTGGGG133950.027.089645245
AAGGGGG116950.026.865658235-239
CGACGCC20403.092282E-1026.681309245
GGAGGAG178600.026.412405245
CCCGGGG59300.025.979147235-239
CCACCCC283750.025.896149245
Produced by FastQC (version 0.11.2)